Hi Shaun, Please download hg19.2bit file from http://hgdownload.cse.ucsc.edu/goldenPath/hg19/bigZips/, and update your tool-data/alignseq.loc file with the following line: seq hg19 PATH_TO_hg19.2bit (tab-separated fields) This will then let the 'fetch sequences' tool to access hg19 sequences. Thanks for using Galaxy, Guru Galaxy team.

From: SHAUN WEBB <swebb1@staffmail.ed.ac.uk> Date: January 27, 2010 9:50:23 AM EST To: galaxy-dev@bx.psu.edu Subject: [galaxy-dev] Storing Genomic Sequences

Hi, we would like to store the new hg19 sequence locally for use with galaxy tools (e.g Fetch Genomic Sequence). I was wondering which files were required to this and where they are located.

Thanks Shaun Webb

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