You are correct about the tools, so the problem is most likely with the
original GTF file. If gene_id is not assigned there correctly, then the
data will not be sorted by gene_id.
Although GTF format is consistent (mostly!) between sources, the actual
content can vary. One example is from UCSC - the GTF format from the
Table browser will have the transcript name assigned to both the gene_id
and the transcript_id tags in the attributes field (f9). Post processing
to extract gene name from the track and swapping it into the GTF file's
gene_id attribute tag would be a necessary pre-processing step before
using the downstream tools with functionality that would use the attribute.
The good news is that you should be able to use Galaxy's Text
Manipulation tools to do whatever file processing you need to do, from
whatever input source you are using, once you have the data content
loaded into your history. Create->save->use a workflow so that you only
have to work out tedious file conversions step-by-step one time.
If you need more help, please let us know and share your history:
Options -> Share or Publish -> Share with a user "jen(a)bx.pus.edu".
ps. It is best to send data questions to galaxy-use mail list to help
the community learn from each other. I am going to forward this answer
there now, since this question has come up a few times recently after
the addition of the new tools.
On 12/1/10 7:00 AM, David Matthews wrote:
Hope you can help, after using cuffdiff on my data using the combined
gtf files from cuffcompare I get the usual list of files back. However,
in the genes tracking file and the genes fpkm files many genes are
listed more than once. My understanding was that cuffdiff was supposed
to amalgamate these into one whole number for that gene id, am I doing