 
            
            
            
            
                14 Aug
                
                    2012
                
            
            
                14 Aug
                
                '12
                
            
            
            
        
    
                6:12 p.m.
            
        Dear All, I have some FASTQ datasets in phred 33 offset, and I have already assinged them Fastqsanger format. Do I need to run FASTQ Groomer on these datasets before I check the data quality by "Fastqc: Fastqc QC" and "FASTQ Trimmer by column" to remove bad nucleotides at 3' end of reads? Should I select "Sanger" as "Input FASTQ quality scores type:" if I need to run Groomer? Thanks. Jianguang Du