14 Aug
2012
14 Aug
'12
8:12 p.m.
Dear All,
I have some FASTQ datasets in phred 33 offset, and I have already assinged them Fastqsanger format. Do I need to run FASTQ Groomer on these datasets before I check the data quality by "Fastqc: Fastqc QC" and "FASTQ Trimmer by column" to remove bad nucleotides at 3' end of reads?
Should I select "Sanger" as "Input FASTQ quality scores type:" if I need to run Groomer?
Thanks.
Jianguang Du