Apologies for the long content..
I seem to be having a discrepancy with fastx_collapser as well. I have converted from s_8_sequence_clipped.fa to s_8_sequence_collapsed.fa fastx_collapser -v -i s8_sequence_clipped.fa -o s8_sequence_collapsed.faInput: 26580941 sequences (representing 212647528 reads) ---> what the..?Output: 3177400 sequences (representing 212647528 reads) In my collapsed file, my top read is: >1-35820208TACCTGGTTGATCCTGCCAGTAG (this is already over my original read count) When I grep -e TACCTGGTTGATCCTGCCAGTAG -c s_8_sequence_clipped.fa 4,503,566 .... Ok, I've reanalyzed a previous dataset and the output is consistent with my previous numbers: fastx_collapser -v -i s3-sequence.fa -o s4-sequence-RETEST.faInput: 36008043 sequences (representing 36008043 reads)Output: 3886503 sequences (representing 36008043 reads) so it doesn't appear to be the toolkit. How are the tools counting "reads" and "sequences" ?Could the format of the headers affect it? hyphens vs underscores? This data set:>ILLUMINA-08A740_0000:8:1:1736:1055#0/1GCGAGCGTAGTTCAATGGTAAAACATCTCCTTGCCAAGGA Previous data set:>GAPC_0034_FC:1:1:1548:1028#0/1AAACTTCATCGTTATCGAGCGA
Thanks From: keneclip@hotmail.com To: galaxy-user@bx.psu.edu Date: Thu, 14 Apr 2011 00:54:05 +0000 Subject: [galaxy-user] regarding read counts from fastx_clipper
Hi, I'm using the fastx_toolkit (v0.0.13) command line scripts. When using fastx_clipper, I get: fastx_clipper -a TCGTATGCCGTCTTCTGCTTG -v -c -l 15 -M 5 -i s_8_sequence.fa -o s_8_sequence_clipped.faClipping Adapter: TCGTATGCCGTCTTCTGCTTGMin. Length: 15Non-Clipped reads - discarded.Input: 227673720 reads.Output: 212647528 reads.discarded 3527200 too-short reads.discarded 725608 adapter-only reads.discarded 10773384 non-clipped reads.discarded 0 N reads. The s_8_sequence.fa file is 2.2Gb, s_8_sequence_clipped.fa file is 1.7Gb.... seems like fastx_clipper is reporting way too many reads in this instance. I also tried without the -M option but same thing. I checked with: wc -l s_8_sequence.fa56918430(divide this by 2 gives 28,459,215 reads) wc -l s_8_sequence_clipped.fa53161882(divided by 2 gives 26,580,941 reads)
There has never been such a discrepancy with this tool. I'm not sure if I'm doing something silly this time round, or somethings changed in my system that's affecting fastx_clipper counting. Heres a couple of lines from input and output: head -n 6 s_8_sequence.fa>ILLUMINA-08A740_0000:8:1:1736:1055#0/1GCGAGCGTAGTTCAATGGTAAAACATCTCCTTGCCAAGGA>ILLUMINA-08A740_0000:8:1:2219:1057#0/1CAAGCGTCGGAGGTTTAGTCTTTCGTATGCCGTCTTCTGC>ILLUMINA-08A740_0000:8:1:2316:1056#0/1TACCTGGTTGATCCTGCCAGTAGTCGTATGCCGTCTTCTG head -n 6 s_8_sequence_clipped.fa>ILLUMINA-08A740_0000:8:1:2219:1057#0/1CAAGCGTCGGAGGTTTAGTCTT>ILLUMINA-08A740_0000:8:1:2316:1056#0/1TACCTGGTTGATCCTGCCAGTAG>ILLUMINA-08A740_0000:8:1:3041:1059#0/1GAAGCTGCGGGTTCGAGCCCCGTCAGTCCCGCCA
Any ideas? Thanks, Ken
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