26 Feb
2013
26 Feb
'13
10:50 p.m.
On Mon, Feb 25, 2013 at 5:09 PM, Mohammad Mohiuddin wrote:
Hi,
... After uploading and processing the data, I will have to BLAST all my sequences using NCBI BLAST but there is no BLAST option in galaxy. How can I do that? And, is there any way to change the default E-value of Galaxy?
Hi Mohiuddin,
The public Galaxy server only offers Megablast (under NGS:Mapping) and only against four specific databases. If you want more options, there are wrappers for the NCBI BLAST+ suite in the Galaxy Tool Shed http://toolshed.g2.bx.psu.edu/view/devteam/ncbi_blast_plus which can be installed in a local copy of Galaxy. If your group, department/university/company has their own Galaxy Instance, speak to your local Galaxy administrator about this.
Regards,
Peter