8 Apr
2013
8 Apr
'13
11:45 p.m.
Hi All,
I have a very basic question. I have RNA-seq datasets of several cell types and want to compare the alternative splicing events between cell types. The reads are 36nt in length. Are these reads long enough to map on the splicing jucntions accurately when I run Tophat with stringent parameters (no mismatch)?
Thanks.
Best,
Jianguang Du