Hi, I currently have read names with the format: @N57638:1:64JU0AAXX:1:1:1057:943 1:Y:0: and would like to change them to the format: @N57638:1:64JU0AAXX:1:1:1057:943/1
I use Manipulate FASTQ, on all reads and set 'Manipulate Reads on:' to 'Name/Identifier', ('String Translate' becomes the only option). I then set the 'From:' field to '1:Y:0:' and the 'To:' field to '/1' (without the literal quotes). I get the following error:
Traceback (most recent call last): File "/home/home/galaxy/software/galaxy-central/tools/fastq/fastq_manipulation.py", line 37, in main() File "/home/home/galaxy/software/galaxy-central/tools/fastq/fastq_manipulation.py", line 25, in main new_read = fastq_manipulator.match_and_manipulate_read( fastq_read ) File "/home/home/galaxy/software/galaxy-central/database/job_working_directory/942/tmpgp13Qy", line 15, in match_and_manipulate_read new_read = manipulate_read( fastq_read ) File "/home/home/galaxy/software/galaxy-central/database/job_working_directory/942/tmpgp13Qy", line 8, in manipulate_read new_read.identifier = "@%s" % new_read.identifier[1:].translate( maketrans( binascii.unhexlify( "313a593a303a" ), binascii.unhexlify( "2f31" ) ) ) ValueError: maketrans arguments must have same length
So, do the From and To fields really need to be the same length? This seems rather strange and unhelpful. Am I doing something wrong?
Many thanks, Graham
Dr. Graham Etherington Bioinformatics Support Officer, The Sainsbury Laboratory, Norwich Research Park, Norwich NR4 7UH. UK