To Whom It May Concern:
I am a new user to Galaxy. In the function of "FASTQ Groomer", I noticed there is an option for "Input FASTQ quality scores type". My question is what different conversions you will do when I choose "Sloexa" or "Illumina 1.3+". I am asking this question because I used to use Maq's sol2sanger (I guess it is just similar to your "Solexa") to convert all data generated by Illumina 1.5. It seems like, based on your options, I should have chosen other conversion (e.g., your "Illumina 1.3+") to convert data generated by Illumina 1.5
Also, it looks like "Sloexa" and "Illumina 1.3+" just differ in the quality score calculation. But, when I use BWA and SAMtools to do mapping and call SNPs, I notice the size of the bam or pileup files are very different between those two different conversions. Also, it looks like even the coverage for some of the bases are different when choosing different conversions.
Can you tell me how the conversion can affect the final result in terms of coverage?
All your help will be greatly appreciated!
-Jianchao Yao