Hello forum, I have ran cuffdiff with two samples, treated vs untreated, and had certain values as expressed in the output (isoform differential expression).
When I ran it again, using a different treated sample, but SAME UNTREATED SAMPLE, the values assigned to the untreated are different.
Why is this if values are calculated from a unique FPKM? This happend for other jobs where I have ran the same untreated vs other treated samples...
Thanks a lot, ib