26 Apr
2011
26 Apr
'11
4:44 p.m.
Hi Experts, I have single end Illumina reads from ChIP-Seq experiment. The files have encoding Illumina 1.5, and the sequence length is 76bp. After basic FastQc I want to map the sequences using Bowtie. My question is: do I need to split my reads (farward and backward) before running mapping tool? In one of Galaxy screen shorts reads are spitted while not in the other. Thank you in advance, F