27 Apr
2011
27 Apr
'11
4:44 a.m.
Hi Experts,
I have single end Illumina reads from ChIP-Seq experiment. The files have encoding Illumina 1.5, and the sequence length is 76bp.
After basic FastQc I want to map the sequences using Bowtie. My question is:
do I need to split my reads (farward and backward) before running mapping tool?
In one of Galaxy screen shorts reads are spitted while not in the other.
Thank you in advance, F