I have a VCF file and I want to filter it for nonsynonymous/ deletion/
insertion seq variations. Once I filter this file and compare between
tumor vs normal samples and then annotate such variations. I believe I can
filter this file using SnpSift and then can annotate with SnpEff, When I
try to use Snsift filter it just says arbitrary expression. Are there rules
how to use expression for a particular filter with in galaxy. If any one
has used SnpSift in galaxy may share their expertise.
Thanks
Kanwar