[galaxy-user] confusion from "Extract Genomic DNA (version 2.2.3) "

Hi Galaxy team,

I recently met two problems when I used " Fetch sequences> Extract Genomic DNA" in Galaxy Main instance.

I wanted to extract the exons according to the coordinates in a GFF3 file from my reference sequences (from history, and I specified the genome build) which are in FASTA format.

But after checking the output, I found:

1. The first base of each extracted exon was missing in the output, so each extracted exon sequence is one nucleotide shorter than the real length.

2. Some extracted exons are correct,but some extracted exons are wrong. The questionable exons could not be found in the corresponding reference. I can not figure out where they are from.

I tried to read the manual/warnings in the page. But I have no idea with my strange output. Could anyone give me some clues,please?

Thanks.

Best,

Miranda