Hi there,
I have two questions regarding alignment using Bowtie:
1. Is there a way to set the Seed Length (-l) to the full length of each read instead of using a single Seed Length for all reads? The seed length is a single input value for all sequences in any
Hi Eric, On 3/19/12 4:13 PM, Eric Guo wrote: particular job.
2. When using m = -1 mode (Suppress all alignments for a read if more than n reportable alignments exist (-m): OFF), will a read be randomly assigned to one of the alignment positions in the genome? Or, will all the alignments for one single read be reported in the final output .bam file.
If alignment are not suppressed, then all reportable hits are in the output. More about Bowtie can be found here: http://bowtie-bio.sourceforge.net/index.shtml Best, Jen Galaxy team
Thanks in advance for your help.
-Eric
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