Hi Eric,
On 3/19/12 4:13 PM, Eric Guo wrote:
Hi there,
I have two questions regarding alignment using Bowtie:
- Is there a way to set the Seed Length (-l) to the full length of each
read instead of using a single Seed Length for all reads?
The seed length is a single input value for all sequences in any particular job.
- When using m = -1 mode (Suppress all alignments for a read if more
than n reportable alignments exist (-m): OFF), will a read be randomly assigned to one of the alignment positions in the genome? Or, will all the alignments for one single read be reported in the final output .bam file.
If alignment are not suppressed, then all reportable hits are in the output.
More about Bowtie can be found here: http://bowtie-bio.sourceforge.net/index.shtml
Best,
Jen Galaxy team
Thanks in advance for your help.
-Eric
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