Hello Ateequr, The quickest way to use new genomes with tools is to use them as a custom reference genome. To do this, load the genome in fasta format into your history and set tool form options to use reference genomes "from the history". Best practices: 1 - double check that the format is strict fasta (all lines wrapped to same length, no extra lines or hidden characters, sequence entries are unique within file). 2 - use the same genome dataset for all steps within an analysis 3 - load using FTP and make certain load is complete http://galaxyproject.org/wiki/Learn/Upload%20via%20FTP Hopefully this helps! Best, Jen Galaxy team On 1/13/12 7:00 AM, Ateequr Rehman wrote:
Dear Galaxy Administrator
I am analysing transcriptome and genome of clinical isolatesStenotrophomonasmaltophilia, would be highly obliged if you could please upload Stenotrophomonas maltophilia K279 and Stenotrophomonas maltophilia R551-3 genome acccesion numbers at NCBI are as following
Stenotrophomonas maltophilia K279 (Accession AM743169)
*Stenotrophomonas maltophilia R551-3*, complete genome <http://www.ncbi.nlm.nih.gov/nuccore/CP001111.1>
Accession: CP001111
Thanking you all in advance Ateequr Rehman
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