Hi all,
Fastq groomer has Solexa or Illumina 1.3+ as an input quality format. I
asked at the sequencing facility about their machine and output and they
said their format was Illumina 1.8+ (the newest). I tried to convert my
fastq file into Sanger by fastq groomer, using Illumina 1.3+ as an input
option and got all reads with quality of around 10... Does it mean that
Galaxy cannot be used on a dataset with 1.8+ encoding or something else was
wrong?
Thanks,
Slon