I have a question about the groomer. Do all Illumina runs need to be groomed, or are there situations where it can be skipped?? (My data says illumina 1.5, so ive been picking input type as illumina 1.3-1.5.) rich ________________________________ From: Jennifer Jackson <jen@bx.psu.edu> To: Ateequr Rehman <ateeqrr@yahoo.com> Cc: "galaxy-user@lists.bx.psu.edu" <galaxy-user@lists.bx.psu.edu> Sent: Wednesday, February 29, 2012 10:57 AM Subject: Re: [galaxy-user] ILLumina 1.9 Hiseq Hello, The input quality score type should be set as "Sanger" for your data. Thanks! Jen Galaxy team On 2/29/12 7:39 AM, Ateequr Rehman wrote:
Dear Glaxy users and admin
I ran my sequence data on FASTQC tool, output says it is Encoding Sanger / Illumina 1.9
now i want to groom my file, but groomer does not have option for 1.9 in "Input FASTQ quality scores type"
any idea which option i should select to grroom my file,
later i want to run Bowtie or Tophat,
Thanks **
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-- Jennifer Jackson http://usegalaxy.org http://galaxyproject.org/wiki/Support ___________________________________________________________ The Galaxy User list should be used for the discussion of Galaxy analysis and other features on the public server at usegalaxy.org. Please keep all replies on the list by using "reply all" in your mail client. For discussion of local Galaxy instances and the Galaxy source code, please use the Galaxy Development list: http://lists.bx.psu.edu/listinfo/galaxy-dev To manage your subscriptions to this and other Galaxy lists, please use the interface at: http://lists.bx.psu.edu/