On Wed, Jun 1, 2011 at 5:02 PM, John David Osborne ozborn@uab.edu wrote:
I noticed that for our new Ilumina data (which generate Sanger format) the FastQ groomer output is identical to the Ilumina FastQ input file.
I was hoping to go ahead and just use the raw FastQ files as input (saving disk space) for computing quality statistics to look at box plots, but it appears that the tool "Compute Quality Statistics" appears to require that the data have been run through FastQ Groomer first.
Is there a way to get around this and is this a bug? I assuming this is some sort of safety measure built into this tool?
-John
If you know your data is already in Sanger FASTQ format, you can say this when uploading the data into Galaxy. Or, use the "pencil" icon to edit the attributes and change the file type. This doesn't change the file itself on disk.
Peter