Hello, This means that your BAM file contains only aligned data. Running another tool that counts up similar statistics, such as "NGS: SAM Tools -> flagstat" could be used to confirm the counts. Best, Jen Galaxy team On 8/12/12 3:00 PM, i b wrote:
Hi, I have used for the first time this tool, picard bam statistic. I have aligned my reads to a custom genome (7904 bp long) and had the following output:
length= 7904 Aligned= 44 Unaligned= 0
Why is unaligned =0.
I had the unaligned =0 also when aligning agaist hg19...
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