Hi, I've been using lastz to map some 454 reads to a reference sequence on galaxy. I'm not clear on what the mapping modes do for the analysis. If I am already setting a cut-off value for the percent identity for reporting matches then what exactly does the mode setting do? In the tutorial you set a roche-454 98% mode based for a re-sequencing example and then set the identity to 90%, what parameters in lastz does the mapping mode change? thanks, H -- Heather Kent Bioinformatics Core Facility Public Health Agency of Canada National Microbiology Laboratories (204)784-7503 Quote of the week: Most people are other people. Their thoughts are someone else's opinions, their lives a mimicry, their passions a quotation. Oscar Wilde (1854 - 1900), De Profundis, 1905