Hi Andrew, My first guess is that perhaps the region is not set correctly? http://wiki.galaxyproject.org/CloudMan/AWS/GettingStarted See " Step 1: One Time Amazon Setup", subsection 2, where region is " set your AWS Region to US East (Virginia)". The image in the wiki for step 1.2 is slightly outdated, instead it will look like this: Please give this a try and let us know if you continue to have issues. Thanks! Jen Galaxy team On 4/8/13 3:56 PM, Thompson, Andrew wrote:
Dear Jen Thanks. I have merged the files and end up with 4 x 47 G fastq files for read mapping to the reference. It seems this is too much data to analyse on the public main instance if the size limit is 250 G? So I tried to set up the cloud option following the screencast (http://screencast.g2.bx.psu.edu/cloud/), but when I search for the current AMI name (861460482541/galaxy-cloudman-2011-03-22) it is not found in the list of community AMIs under Amazon's EC2 Management Console. Any ideas why this is not working? regards Andrew
-- Jennifer Hillman-Jackson Galaxy Support and Training http://galaxyproject.org