12 Jul
2011
12 Jul
'11
4:02 p.m.
Hi I am sequencing a bacterial genome and have assembled my Illumina reads (40 bp single) using bowtie with a reference genome. This generated a sam file. I would like to obtain a listing of the open reading frames from the bacterial genome and the corresponding genes that they are most similar to. Can you please give the tools/steps necessary to do this?
many thanks
Jo