15 Aug
2012
15 Aug
'12
6:59 p.m.
Dear All,
I have some paired-end datasets to be analyzed, but I am not sure about their Mean Inner Distance between Mate Pairs.
Can I convert these paired-end datasets into single-end ones and use them as single-end dataset as follows?
1) Use the tool "Manipulate FASTQ" to convert the sequence of reverse reads into its reverse-complement counter part, so that all of the reverse reads actually become forward reads.
2) run Tophat on the manipulated datasets as single-end ones.
Thanks.
Jianguang