[galaxy-user] cufflinks set parameters

Hi,   It's the first time for me to use cufflinks in galaxy, two choices confused me as follows: Perform Bias Correction: No Yes Bias detection and correction can significantly improve accuracy of transcript abundance estimates. Set Parameters for Paired-end Reads? (not recommended): No Yes what is the Bias correction and how does it work? And for "set parameters for paired-end reads? (not recommended)" ,what would be important difference between recommended and not recommended? Thanks a lot! dongdong