It's the first time for me to use cufflinks in galaxy, two choices confused me as
Perform Bias Correction:
Bias detection and correction can significantly improve accuracy of transcript abundance
Set Parameters for Paired-end Reads? (not recommended):
what is the Bias correction and how does it work? And for "set parameters for
paired-end reads? (not recommended)" ,what would be important difference between
recommended and not recommended?
Thanks a lot!