Hi Cai,
The datatype for your dataset must be set to 'fastqsanger' for the BWA wrapper to recognize it; it is probably simple 'fastq' in your history. You can manually set this attribute, by clicking on the pencil icon for the history item and selecting the correct option under the relevant dropdown menu. You can also use the FASTQ Groomer (sanger to sanger) or declare the fastq datatype variant when uploading. Please let us know if we can provide additional assistance.
Thanks for using Galaxy,
Dan
On Jan 4, 2012, at 4:44 AM, Cai Shaojiang wrote:
Dear friends,
We just installed a fresh galaxy on the server. Now when we try to run BWA following this tutorial: http://wiki.g2.bx.psu.edu/Admin/Training/Galaxy%20Admin%20Tutorial.
It keeps telling that "FASTQ file: History does not include a dataset of the required format / build Must have Sanger-scaled quality values with ASCII offset 33"
But we indeed have got the fastq file from http://lava.mathcs.emory.edu/outgoing/data/phiX174_reads.fastqsanger. And I also tried to upload a fastq file from my local disk. Still not working. So I am guessing maybe the default bwa wrapper is something wrong? Thanks.
Yours: Cai
-- Cai Shaojiang Department of Information Systems, School of Computing, National University of Singapore Tel: +65-65167355 ___________________________________________________________ The Galaxy User list should be used for the discussion of Galaxy analysis and other features on the public server at usegalaxy.org. Please keep all replies on the list by using "reply all" in your mail client. For discussion of local Galaxy instances and the Galaxy source code, please use the Galaxy Development list:
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