the file looks good to me (illumina 1.8 because of the ?). I might have misunderstood the error message. If it comes from galaxy when you try to use this file, then you have not specified the correct file format to galaxy. You have to change its type to fastqsanger by clicking on the pencil.
HTH, ido
On Aug 27, 2013, at 5:05 PM, "Law, Michael J." lawmj@rowan.edu wrote:
Of course....Here is an example: @HWI-ST1160:394:C1J65ACXX:7:1101:1520:2235 1:N:0:TTAGGC CTGACATAGTCTGGTGCGGCAACAATCATTCTTGCCATGAGCCCACCTAG
@CCDFFFDHDHHGIFHIIJJJJJIGIJ9FIHIBHIIECE@EGGJGHJJBH @HWI-ST1160:394:C1J65ACXX:7:1101:1946:2192 1:N:0:TTAGGC GCGGATATTGGTACAATAGGAGCACCGTCAGCAATGGTACCTCTGATGAA
@CCFDDFFHHHHHJIJJJJJHIJGJJJJJGIJIJJJJ?DGGIJJJGIIIJ @HWI-ST1160:394:C1J65ACXX:7:1101:1851:2248 1:N:0:TTAGGC CTTTGAGTGAACCTCGTCCGGTACTGCTTTTTATTGCCATGTATTATGTG
@CCFDEFDFHHGHJJJHJJJJCGHIJJJIJJI@FIJJJHIJIIIIDGGFF @HWI-ST1160:394:C1J65ACXX:7:1101:2249:2219 1:N:0:TTAGGC TCCTCAACCAGTCTTCCTTTTCATCATTTGACTCCAGTGCAGTGAAATCG
CCCFFFFFGHHFHJJJJJJJJJIJIIIJIEHHGIIFHHIIIJHGIJJIJJ Also, whenever I send an email, it says I am not a member, but I have a login (clearly since I have used ftp for data upload). How do I fix this? Thanks so much, Mike
Michael Law lawmj@rowan.edu
On Aug 27, 2013, at 4:44 AM, Ido Tamir wrote:
You should add 10 lines from one of your files. Then its easier to understand the problem.
best, ido
On Aug 26, 2013, at 5:17 PM, "Law, Michael J." lawmj@rowan.edu wrote:
Hello, I am completely new to using galaxy. I have a quick question. I have uploaded my fastq files generated from my experiment for alignments using bowtie. I try to set up the alignment, only to receive the error message that I don't have any sequences with ASCII encoded quality scores. However, when I contact my sequencing facility, they say that the scores are present in the files and that it may be an issue with galaxy. Any help you can provide would be appreciated! Thanks, Mike
Michael Law lawmj@rowan.edu
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