Dear Andrea:
Many apologies for a delay answering this. We are currently in the process of shifting genotyping pipelines to use VCF format, which, combined with a set of VCF manipulation utilities, will make it quite easy to perform operations you request. These should be ready for use by the end of Summer.
Thanks for using Galaxy,
anton galaxy team
Anton Nekrutenko http://nekrut.bx.psu.edu http://usegalaxy.org
On May 18, 2011, at 7:39 PM, Andrea Edwards wrote:
Hello
I was wondering if there was anything available within galaxy that would let you do the following with pileup files:
- filter for homozygous SNVs (i.e. that do not contain the reference genome allele in the genotype)
- compare the pileup files for 2 (or more) individuals to find SNVs unique to each individual and to further limit this to homozygous SNPs unique to each individual
- compare the pileup files for 2 (or more) individuals to find shared SNVs and to further limit to this to shared SNVs where the individuals have different alleles (rare as would assume triallelic snv) and then group the individuals according to the allele for each SNV.
I could only see the filter pileup tool but nothing for comparing pileup files
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