Hello Di, The good way to load data in a local instance is through the Data Library function: http://wiki.g2.bx.psu.edu/Admin/Data%20Libraries http://wiki.g2.bx.psu.edu/Admin/Data%20Libraries/Uploading%20Library%20Files If you run into trouble, you will probably want to switch over to the galaxy-dev@bx.psu.edu mailing list to post your question. That list has a focus on local instance support and give your query the best visibility for a quick response. Take care, Jen Galaxy team On 7/23/12 4:55 PM, Di Nguyen wrote:

Dear all,

I am having a problem uploading my RNA-seq files into my local instance. What would be the easiest way to set this up? I already setup libraries under Admin but cannot upload file directly, perhaps due to the size limit of the browser.

1.FTP? Please give me a step by step instruction. I got no problem setting up Fizilla against Main Galaxy. I looked at the wiki page but not quite clear on what to do

2.Direct upload from local file path (limit by the size of the file << 2Gb, which is not practical?

My utmost appreciation,

Di Nguyen U of Washington, Seattle, WA ___________________________________________________________ The Galaxy User list should be used for the discussion of Galaxy analysis and other features on the public server at usegalaxy.org. Please keep all replies on the list by using "reply all" in your mail client. For discussion of local Galaxy instances and the Galaxy source code, please use the Galaxy Development list:

http://lists.bx.psu.edu/listinfo/galaxy-dev

To manage your subscriptions to this and other Galaxy lists, please use the interface at:

http://lists.bx.psu.edu/

-- Jennifer Jackson http://galaxyproject.org