I posted this question in Dev- list so re-posting to correct list with some additional information. I have paired end sequencing files on which I would like to call SNPs compared to databases well check sequence variation among samples. I dont have access to any local galaxy instance. My question are- 1. Is there a way that I can upload these large files to galaxy and analyze them then delete? 2. Is there a work flow to call SNPs and analyze and annotate SNPS in Galaxy, If some one know about a work flow summarized by some one in addition to Galaxy list that will be bonus. Thanks Kanwar