Hello Jianguang, Given this information, you will not need to run FASTQ Groomer, but simply proceed with Fastqc and FASTQ Trimmer. Best, Jen Galaxy team On 8/14/12 11:12 AM, Du, Jianguang wrote:

Dear All,

I have some FASTQ datasets in phred 33 offset, and I have already assinged them Fastqsanger format. Do I need to run FASTQ Groomer on these datasets before I check the data quality by "Fastqc: Fastqc QC" and "FASTQ Trimmer by column" to remove bad nucleotides at 3' end of reads?

Should I select "Sanger" as "Input FASTQ quality scores type:" if I need to run Groomer?

Thanks.

Jianguang Du

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