Andy: This is an issue we are aware of. To workaround is: - click on a pencil icon within the pileup dat set you nee to filter - middle pane on the interface will change - click on "datatype" tab - select "pileup" in "New datatype" - click save Filter pileup will now recognize you dataset. Thanks and sorry for late reply. anton Anton Nekrutenko http://www.galaxyproject.org On Feb 22, 2013, at 3:58 AM, Andrew South wrote:

NGS: SAM Tools

I have generated a simple 6 column pileup from my BAM file but when I try to use the Filter Pileup tool it doesn’t recognise my pileup file, I receive the message “History does not include a dataset of the required format / build”. Build is hg19. Curiously in the same history I have ran the tool through a workflow without a hitch.

Any advice to successfully filter my pileup would be gratefully received.

Many thanks,

Andy

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