Hi Mathew, Please see the tool 'NGS: QC and manipulation -> FASTQ Trimmer by column'. Make sure your data is in ".fastqsanger" format first - either assigned or through grooming: See "FASTQ": http://wiki.galaxyproject.org/Support#Dataset_special_cases Take care, Jen Galaxy team On 5/1/13 7:29 AM, Mathew Bunj wrote:

Is it possible to trim certain number of bases from 3' end with taking into consideration quality of reads. To explain it further, I want to remove 10bases from 3 ' end of all reads and keep first forty.

Thanks

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