Hello, The Megablast htgs, nt, and wgs databases are in the process of being updated to the latest NCBI releases and are expected to be available by tomorrow morning (possibly sooner). Should you wish to continue your analysis using the prior versions, these are available through our rsync server for use in a local production or cloud Galaxy. https://wiki.galaxyproject.org/Admin/UseGalaxyRsync http://getgalaxy.org http://usegalaxy.org/cloud Also posted to Galaxy Biostar: https://biostar.usegalaxy.org/p/7335/#7340 Best, Jen Galaxy team On 4/27/14 4:25 AM, Scott W. Tighe wrote:

Jennifer

I am megablasting a simple 500,000 line dataset that is certainly in galaxy fasta.

For a week i have been seeing numerous errors. So i have reprocesed the data multiple times.

The error message is "could not find specified database directory"

Is there an alternative approach? I did try ncbi directly and that failed too. They say they have a database issue

Scott Tighe

Core Laboratory Research Staff Advanced Genome Technologies Core Deep Sequencing (MPS) Facility Vermont Cancer Center 149 Beaumont Ave University of Vermont HSRF 303 Burlington Vermont USA 05045 802-656-AGTC 802-999-6666 (cell)

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