Hi Amit, The workflow requires the input data to be in 'fastqsanger' format before being able to run. The files you uploaded from S3 are already in the correct format but this is most likely not set correctly in the metadata. So, click on the pencil icon for each of the datasets in your history and edit the data type by setting it to 'fastqsanger'. Save the changes and try running the workflow. It should work fine then. For future reference, if you decide to upload the rest of the files from the screencast/heteroplasmy study, you can choose the fastqsanger type right on the data upload form and can thus avoid this subsequent step. Enis On Mon, May 9, 2011 at 5:23 PM, Amit Indap <indapa@gmail.com> wrote:

Hi Galaxy,

I am a newbie to Amazon EC2 and have been carefully following the steps in the screencast. I am able to upload two fastq files from the s3 bucket: http://s3.amazonaws.com/heteroplasmy/F4-bM4-1.fastq http://s3.amazonaws.com/heteroplasmy/F4-bM4-2.fastq

I am also able import the published workflow

http://s3.amazonaws.com/heteroplasmy/Galaxy-Workflow-mt_analysis_0.01_strand...

But when it comes to running it, I cannot select the fastq file from the drop down menu. I am able to view them on my GC instance, since I imported them successfully, but am at a loss as to why I can't select them from the drop down menu in the workflow to begin their alignment. Is it something to do with my security group settings in setting up the EC2 instance? Any assistance you can provide would be great!

Thanks, Amit

-- Amit Indap ___________________________________________________________ The Galaxy User list should be used for the discussion of Galaxy analysis and other features on the public server at usegalaxy.org. Please keep all replies on the list by using "reply all" in your mail client. For discussion of local Galaxy instances and the Galaxy source code, please use the Galaxy Development list:

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