Hello Cosmas, When you sent the data over to UCSC, did you save it there into a Session? If not, then the custom track data will expire in 48-72 hrs (as all custom track data does, not just those derived from Galaxy). The solution is to reload from Galaxy into the UCSC Browser each time you want to use it there or to create a Sessions account at UCSC and save your custom tracks into it when you want to work with them for longer periods of time. Here is a link into the UCSC Sessions help page to get you started: http://genome.ucsc.edu/goldenPath/help/hgSessionHelp.html Hopefully this helps! Jen Galaxy team On 11/24/10 8:52 PM, Giallourakis, Cosmas P.,M.D. wrote:

I analyzed data and for several days could see data loaded as custom track on USCSC browser, but no wall of the sudden track is there but EMPTY

Can someone please explain what is going on? Thx Cosmas Giallourakis, MD Assistant Professor of Medicine Harvard Medical School Massachussetts General Hospital Gastrointestinal Unit 55 Fruit Street Boston, MA 02114 clinical office (617)-726-2026

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