Hello Alicia, Would you please share with me the history on the public Main server that you are having trouble downloading the data from? Use "Options -> Share or Publish", generate the link or add my email as a shared user, and email the link or name of the history back to me directly. Thanks, Jen Galaxy team On 1/13/12 1:28 AM, Poh Leng Chong wrote:

Hi,

I run Barcode Splitter (from public Galaxy server) and am unable to download the text files generated by the barcode splitter, got an error message which says '

Could not find 'FASTQ_Groomer_on_data_1__V1.txt' on the extra files path /galaxy/main_pool/pool2/files/003/610/dataset_3610002_files/FASTQ_Groomer_on_data_1__V1.txt.

The run was Ok (green colour) but yet i was unable to download the text files. Could you please advise?

Also, I wonder if you could help regarding the local Galaxy.. I used VirtualBox to install the local copy, and installed according to the instructions as in the website, command as "hg clone https://...." The setup was smooth and went OK. The problem starts when I run the local Galaxy using real data. I could upload the files, but have problems with the fastQC and barcode splitter, I could not proceed any further since these two operations could not be run...The error messages are:

FastQC An error occurred running this job: Traceback (most recent call last): File "/home/Desktop/galaxy-dist/tools/rgenetics/rgFastQC.py", line 141, in<module> assert os.path.isfile(opts.executable),'##rgFastQC.py error - cannot find executable %s' % opts.executable AssertionError

Barcode Splitter An error occurred running this job: /home/Desktop/galaxy-dist/tools/fastx_toolkit/fastx_barcode_splitter_galaxy_wrapper.sh: line 65: fastx_barcode_splitter.pl: command not found

gzip: stdout: Broken pipe

Can anyone help me please? I installed the local Galaxy but could not proceed further. I then used the public Galaxy but could not download the files....

Hope to hear from you. Thanks very much.

Best regards

Alicia ___________________________________________________________ The Galaxy User list should be used for the discussion of Galaxy analysis and other features on the public server at usegalaxy.org. Please keep all replies on the list by using "reply all" in your mail client. For discussion of local Galaxy instances and the Galaxy source code, please use the Galaxy Development list:

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