It turns out that your artificial test is a bit too artificial. In order to display a coverage plot, Trackster converts reads in a BAM to BigWig using a two step process:

(1) BAM to bedgraph; (2) bedgraph to bigwig

Your super simple example generates an empty file in step 1 because your single read does not map to Araly1, and the tool used in step 2 (bedGraphToBigWig) fails with the error that you're seeing. This is a corner-case bug, and I've create a card for this so you can track its resolution: https://trello.com/c/kMFUNawL

Best, J.

On Oct 30, 2013, at 5:44 PM, "Guest, Simon" simon.guest@agresearch.co.nz wrote:

I'm having problems getting Trackster working on my own Galaxy instance, so I thought I would check on the usegalaxy public server.

However, I'm getting the same Trackster Error: needLargeMem: trying to allocate 0 bytes (limit: 100000000000) that was reported on this list in July, but there was no followup: http://user.list.galaxyproject.org/Trackster-Error-td4655737.html

My history is at https://usegalaxy.org/u/simon-guest/h/trackster-error

This is just an artificial test I made using a fragment of a reference genome, but I thought it should work OK.

Any clues?

cheers, Simon ___________________________________________________________ The Galaxy User list should be used for the discussion of Galaxy analysis and other features on the public server at usegalaxy.org. Please keep all replies on the list by using "reply all" in your mail client. For discussion of local Galaxy instances and the Galaxy source code, please use the Galaxy Development list:

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