16 Nov
2013
16 Nov
'13
2:10 p.m.
Without details about your incoming data rate and size, it's very difficult to answer this question. I suggest taking some of your data, running it on our public server (usegalaxy.org), and seeing how long it takes. RNA-seq tools (Tophat, Cufflinks, etc.) use 4 cores for a job, so you can estimate how many cores you might need (as well as estimate disk space).
I was mistaken: RNA-seq tools use 8 cores on our public server.
Best, J.