Hi Nicola It happens with both versions available on the main toolshed. We originally had the package from bhaas, latest revision from 2012 and yesterday I installed the one from biomonika which requires package_trinityrnaseq_2013_08_14 (which in turn downloads a copy of trinity). Both have the same problem. Since a None value for the strand-spec. parameter means that the parameter is ignored (in the Cheetah code) I would think that the issue does not go down to the tool level (I can run Trinity on the command line just fine without specifying the strand-spec.) Thanks Christian On 08/05/15 17:51, Nicola Soranzo wrote: Hi Christian, was the trinityrnaseq tool installed from the Tool Shed? Which repository? Just to check this is not a tool problem. Cheers, Nicola Il 08.05.2015 10:44 Christian Schudoma (TSL) ha scritto: Hi all, I have encountered a problem when trying to run trinityrnaseq (Galaxy Tool Version 0.0.1) from Galaxy. Leaving the strand-specificity input parameter as "None" will prevent the form from being submitted. Instead, the strand-specificity input box will be highlighted in red and I'm asked to "Please verify this parameter." Before the last update we were running a very very old instance of Galaxy (I think the update before the last was in 2013) from which we could submit trinity-jobs just fine. I found a commit from Aysam from late 2014 which does something in the immediate vicinity of the "Please verify this parameter" - message. (https://bitbucket.org/galaxy/galaxy-central/commits/bda732e549b7/), which I am guessing could have something to do with it. Anyone got any ideas regarding this? Any help would be appreciated. Thanks Christian -- Dr. Christian Schudoma Bioinformatics Support Officer Bioinformatics Group The Sainsbury Laboratory Norwich Research Park Norwich NR4 7UH United Kingdom ****************************** +44 (0) 1603 450 601 christian.schudoma@tsl.ac.uk<mailto:christian.schudoma@tsl.ac.uk> twitter: @cpt_bwt Connetti gratis il mondo con la nuova indoona: hai la chat, le chiamate, le video chiamate e persino le chiamate di gruppo. E chiami gratis anche i numeri fissi e mobili nel mondo! Scarica subito l’app Vai su https://www.indoona.com/

On Fri, May 8, 2015 at 6:55 PM, Nicola Soranzo <nsoranzo@tiscali.it> wrote:

The problem is not in the Cheetah code, which does not get executed because of the validation error.

I think this a Galaxy bug which tries to validate the parameter values but interprets the value "None" of the library_type param as "no option selected".

Nicola

I think this is due to the Python special None object being turned into the string "None" at some point in the Cheetah template code. It may be possible to avoid this by a case variant, e.g. "NONE" or "none", but it may be safer to avoid this special word :( Peter