Re: [galaxy-user] Running Tophat

I have shared my hisotry with you yesterday, can you help me to take a look to see what is the problem for my tophat analysis? Thank you very much!

Ying,

Unfortunately, your job is too large for our cluster. Our cluster has a limit of 250GB of disk space, and Tophat is exceeding this limit while running your job (Tophat generates many intermediary files when running, including a very large SAM file).

Your best bet is to set up and use our cloud instance ( https://bitbucket.org/galaxy/galaxy-central/wiki/cloud ); otherwise, if you have enough resources locally, you can set up a local instance ( https://bitbucket.org/galaxy/galaxy-central/wiki/GetGalaxy ) .

Thanks, J.