Hi galaxy dev team:
I just installed galaxy on my ubuntu box and tried to do RNA-seq analysis according to Jeremy's excellent tutorial
http://main.g2.bx.psu.edu/u/jeremy/p/galaxy-rna-seq-analysis-exercise
however, I encountered following error messages when I was trying to execute Cufflinks in galaxy..
Dataset generation errors
Dataset 121: Cufflinks on data 92: gene expression
Tool execution generated the following error message:
Error running cufflinks. [Errno 2] No such file or directory: 'transcripts.gtf'
The tool produced the following additional output:
cufflinks v1.0.3
cufflinks -q --no-update-check -I 300000 -F 0.050000 -j 0.050000 -p 4 -b
how should I solve this problem?
Thanks in advance.
Best Regards,
John Wu