My best guess is that you are using bias correction but do not have the needed reference
genome(s) for the builds that you want to use. See this page for instructions about
setting up HTS tools; in particular, you'll need to set up the sam_fa_indices.loc
On Jun 9, 2011, at 4:44 AM, 吳正華 wrote:
Hi galaxy dev team:
I just installed galaxy on my ubuntu box and tried to do RNA-seq analysis according to
Jeremy's excellent tutorial
however, I encountered following error messages when I was trying to execute Cufflinks in
Dataset generation errors
Dataset 121: Cufflinks on data 92: gene expression
Tool execution generated the following error message:
Error running cufflinks. [Errno 2] No such file or directory: 'transcripts.gtf'
The tool produced the following additional output:
cufflinks -q --no-update-check -I 300000 -F 0.050000 -j 0.050000 -p 4 -b
how should I solve this problem?
Thanks in advance.
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