I fixed it, Thanks. It was because I aligned to different genomes in BWA and GATK steps
2012/4/3 Lilach Friedman lilachfr@gmail.com
Hi Galaxy Team, I'm trying to use GATK DepthOfCoverage, and to give a BAM file of alined results as input.
My steps were:
- BWA alignment
- SAM-to-BAM
- rmdup.
when I'm trying to give 3 (rmdup results) as an input to GATK DepthOfCoverage, an error appears: " Sequences are not currently available for the specified build."
What did I do wrong?
Many thanks! Lilach