Dear all,
I am a Phd student working on chicken genomics, with limited experience in the bio-informatics field. I performed an RNA-Seq experiment with single end 50 bp reads to find differential gene expressions between different groups. I have mapped this data with Tophat. Does anyone know how I can get the raw data count out of galaxy in a tab-delimited file? I would like to perform a statistical analysis in other software, without the RPKM normalization that cufflinks offers. Thank you very much.
Have a nice day.
Yours Sincerely, Els
--- Ir. Els Willems KU Leuven Department of Biosystems Division Livestock - Nutrition - Quality Laboratory of Livestock Physiology Kasteelpark Arenberg 30 bus 2456 B - 3001 Heverlee T (+32) 016 32 17 29 F (+32) 016 32 19 94