I reload my data with defined format type fastqsanger and it works now.
Thank you so much for your help.
On Thu, Feb 13, 2014 at 6:06 PM, Jennifer Jackson <jen(a)bx.psu.edu> wrote:
I can't comment about a PC (I use a MAC), but this shouldn't matter. If
you are using a local Galaxy (http://getgalaxy.org
), most tools will follow the same usage
rules as when implemented on the public Main Galaxy instance at
, the Galaxy CloudMan ami, and the bulk of other
public Galaxy instances, at least for common tools.
Exceptions would be if the tool(s) were specifically modified to function
differently. Galaxy is open source and variations exist. The tool form of
any alternate version (of a tool named the same) will likely note what
modifications have been made. Plus you can always contact the other public
instance hosts, if you have usage problems.
On 2/13/14 2:26 PM, Gang Wang wrote:
By the way, can you use detect the fastq file using trimmer by column
with your PC. Thanks a lot.
On Thu, Feb 13, 2014 at 1:14 PM, Björn Grüning <bjoern.gruening(a)gmail.com>wrote:
> Hi Wang,
> please check if your fastq file is associated with the correct fastq
> format, fastqsanger probably.
> > Hi,
> > I just notice that NGS FASTQ Trimmer by column can't detect the fastq
> > file I loaded. Anyone knows why. thanks a lot.
> > On Fri, Jan 24, 2014 at 11:00 AM,
> > <galaxy-user-request(a)lists.bx.psu.edu> wrote:
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> > Today's Topics:
> > 1. Re: Creating a Trackster visualisation from a
> > reference in
> > your history (Jeremy Goecks)
> > Message: 1
> > Date: Thu, 23 Jan 2014 15:49:44 -0500
> > From: Jeremy Goecks <jgoecks(a)email.gwu.edu>
> > To: graham etherington (TSL)
> > <graham.etherington(a)sainsbury-laboratory.ac.uk>
> > Cc: galaxy-user(a)lists.bx.psu.edu
> > Subject: Re: [galaxy-user] Creating a Trackster visualisation
> > from a
> > reference in your history
> > Message-ID:
> > <C3382F38-D243-4979-B6A7-BA13EF161EE5(a)email.gwu.edu>
> > Content-Type: text/plain; charset="windows-1252"
> > > Is it possible to create a custom build and use it to view a
> > SAM file without adding the .len and .2bit files in to the
> > Galaxy file system as an administrator?
> > Yes, it definitely is.
> > > If so, what am I doing wrong?
> > This is a Galaxy bug which has been fixed in this commit:
> > We have a release coming up, so this fix will be included in
> > the release and will make it to our public server soon. In the
> > meantime, note that you can use the genome fasta file rather
> > than the len file to create a custom build and everything
> > should work.
> > Thanks,
> > J.