Hi Yuval, You should be able to retrieve RNA co-ordinates using the interface to UCSC genome browser under 'Get data' section on Galaxy. For information on which tracks and tables to choose, please refer these links which contain answers to this topic from UCSC mailing list: http://www.soe.ucsc.edu/pipermail/genome/2007-August/014299.html http://www.soe.ucsc.edu/pipermail/genome/2007-May/013491.html http://www.soe.ucsc.edu/pipermail/genome/2006-April/010436.html Thanks for using Galaxy, Guru Galaxy team. On Jan 27, 2010, at 11:59 AM, Kluger, Yuval wrote:

Hi,

I would appreciate any suggestion how one can use Galaxy to obtain coordinates of human and mouse of all known tRNAs. Similarly how one can do that for other types such as miRNAs and other small RNAs .

Thanks,

Yuval

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Guruprasad Ananda Graduate Student Bioinformatics and Genomics The Pennsylvania State University