Thanks Brad, Sorry it has taken me so long to respond. I had a meeting sort of day. I started another instance to recreate what is happening to me in more detail. I hope this helps. I tried to color code things so you could follow everything more easily. This is what I have when I start up Galaxy through BioCloudCentral before I import any files... Get cloud support with Ubuntu Advantage Cloud Guest http://www.ubuntu.com/business/services/cloud ubuntu@ip-10-44-78-218:~$ df -h Filesystem Size Used Avail Use% Mounted on /dev/xvda1 20G 13G 6.1G 68% / udev 8.4G 4.0K 8.4G 1% /dev tmpfs 3.4G 644K 3.4G 1% /run none 5.0M 0 5.0M 0% /run/lock none 8.4G 0 8.4G 0% /run/shm /dev/xvdb 404G 201M 383G 1% /mnt After starting to import data (pasted https://s3.amazonaws.com/thunnus/BluefinAQ30_shuffled.fastq), it starts filling up immediately Filesystem Size Used Avail Use% Mounted on /dev/xvda1 20G 19G 0 100% / udev 8.4G 4.0K 8.4G 1% /dev tmpfs 3.4G 660K 3.4G 1% /run none 5.0M 0 5.0M 0% /run/lock none 8.4G 0 8.4G 0% /run/shm /dev/xvdb 404G 201M 383G 1% /mnt /dev/xvdg1 700G 654G 47G 94% /mnt/galaxyIndices /dev/xvdg2 10G 1.7G 8.4G 17% /mnt/galaxyTools /dev/xvdg3 500G 81M 500G 1% /mnt/galaxyData